Fingerprinting and validation of a LC-DAD method for the analysis of biflavanones in Garcinia kola-based antimalarial improved traditional medicines

Auteur(s): Tshisekedi Tshibangu Pascal, Mutwale Kapepula Paulin, Kabongo Kapinga Marie Joe, Kabika Lupona Henriette, Ngombe Kabamba Nadège, Kalenda T. Dibungi Pascal
Nom de la revue/Journal: Journal of pharmaceutical and biomedical analysis
Mois: septembre
Volume: 128
Année: 2016
pages: 382-390

Résumé

African populations use traditional medicines in their initial attempt to treat a range of diseases. Nevertheless, accurate knowledge of the composition of these drugs remains a challenge in terms of ensuring the health of population and in order to advance towards improved traditional medicines (ITMs). In this paper chromatographic methods were developed for qualitative and quantitative analyses of a per os <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/antimalarial-agent">antimalarial</a> ITM containing Garcinia kola.The identified analytical markers were used to establish <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/thin-layer-chromatography">TLC</a> and HPLC fingerprints. G. kola seeds were analysed by HPLC to confirm the identity of the extract used by the Congolese manufacturer in the ITM. The main compounds (GB1, GB2, GB-1a and Kolaflavanone) were isolated by preparative TLC and identified by UPLC–MS and NMR. For the quantification of the major compound GB1, a simple and rapid experimental design was applied to develop an LC method, and then its validation was demonstrated using the total error strategy with the accuracy profile as a decision tool. The accurate results were observed within 0.14–0.45 mg/mL range of GB1 expressed as naringenin. The extracts used in several batches of the analysed oral solutions contained GB1 (expressed as naringenin) within 2.04–2.43%.Both the fingerprints and the validated LC-DAD were found suitable for the <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/quality-control">quality control</a> of G. kola-based raw material and finished products, respectively.